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News — gel imaging

Measuring emission spectra for the blue LED transilluminator

gel imaging transilluminator

Measuring emission spectra for the blue LED transilluminator

Our blue LED transilluminators use a 470 nm LED array in combination with blue and amber acrylic filters for viewing DNA bands in agarose gels stained with DNA dyes. Images of gels stained with Greenview, GelGreen and others can be seen in previous posts on our archived site here:  http://www.archive.iorodeo.com/blogs?tid=201 We recently used a Spectrophotometer to measure the transmission spectra of the blue and amber filters used in our transilluminators. In addition we also measured the emission spectra of our blue LED transilluminators with the filters in place.  We used the Ocean Optics USB2000 spectrophotometer for the measurements shown below.  Transmission spectra of the blue acrylic filter...

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Using an imaging enclosure for photographing gels

gel imaging imaging enclosure transilluminator

Using an imaging enclosure for photographing gels

Photographing gels is an important step in research projects that involve any kind of visual or quantitative DNA analysis by electrophoresis. Decent gel imaging requires a fairly simple set-up of a light-tight box with a transilluminator, camera and suitable filters. In research, teaching or DIYBio labs, finding a suitable light-tight box can be the most challenging part of this set-up. We have designed a simple and cheap imaging enclosure (i.e. light-tight box) which works really well for imaging gels with transilluminators. The enclosure is made from machined 0.25" thick black ABS parts (design files are open source and on our...

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