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Chronoamperometry with glucose test strips

Chronoamperometry with glucose test strips

In this electrochemistry experiment we used the Rodeostat potentiostat to measure glucose in liquids using commonly available glucose test strips. Glucose test strips are a type of biosensor - they convert biochemical reactions to an electrochemical signal. Each glucose test strip contains the enzyme glucose oxidase (GOx) and an electron transfer mediator. On the surface of the test strip, glucose oxidase converts glucose to gluconic acid and hydrogen peroxide, transferring two electrons to the flavin (FAD) group in glucose oxidase which is temporarily reduced (FADH2). Enzymes do not readily exchange electrons directly with an electrode, so a mediator is required to shuttle electrons from FADH2...

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Cyclic voltammetry with the Rodeostat

electrochemistry potentiostat rodeostat

Cyclic voltammetry with the Rodeostat

In a previous blog post we explored the irreversible redox reaction of ascorbate using the Rodeostat potentiostat. In this blog post we carried out a cyclic voltammetry experiment with ruthenium hexamine Ru(NH3)6 a reversible redox couple.  An example voltammogram of this redox couple is demonstrated in the Pine electrode literature and we have previously described this experiment using the Cheapstat potentiostat. Here we have repeated this cyclic voltammetry experiment using the Rodeostat potentiostat. A Pine screen printed carbon electrode was also used.  Equipment Rodeostat with screen printed adapter (ver A) Pine screen printed carbon electrode Micropipettes Microcentrifuge tubes Reagents 1) 0.1M KCl  To prepare 500 mL...

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Open source potentiostat features

electrochemistry potentiostat rodeostat

Open source potentiostat features

The Rodeostat (previously known as the Potentiostat Shield) is our newly designed open source potentiostat. We have received a number of questions regarding how the new potentiostat differs from the popular Cheapstat. In this blog post we give a quick overview of some of the main differences between the two open source potentiostats.  Voltage Ranges Rodeostat:  +/- 1, 2, 5, and 10V  Cheapstat:  -0.8 to 1.6V Current Ranges Rodeostat: +/- 1, 10, 100, and 1000uA  Cheapstat: +/- 10 and 50uA  Analog Input/Output Resolution Rodeostat: 12-bit analog output for each voltage range and 16-bit analog input for each current range (13-bits usable) Cheapstat: approx. 2400 values for analog output and approx....

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Testing a lead colorimetric assay

colorimeter lead

Testing a lead colorimetric assay

In this blog post we describe our initial testing of a colorimetric kit for measuring lead in drinking water. In 2015, a Virginia Tech research team found that over 40% of the homes in Flint, MI had elevated lead water levels with 25% of homes well above the EPA 15 ppb maximum contamination level. The levels of lead in drinking water should be zero or undetectable. The Flint water crisis was widely reported in the news and lead contamination of drinking water is still an ongoing public concern. The goal of this project was to find an affordable and easy-to-use lead test for measuring lead in...

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Measuring emission spectra for the blue LED transilluminator

gel imaging transilluminator

Measuring emission spectra for the blue LED transilluminator

Our blue LED transilluminators use a 470 nm LED array in combination with blue and amber acrylic filters for viewing DNA bands in agarose gels stained with DNA dyes. Images of gels stained with Greenview, GelGreen and others can be seen in previous posts on our archived site here:  http://www.archive.iorodeo.com/blogs?tid=201 We recently used a Spectrophotometer to measure the transmission spectra of the blue and amber filters used in our transilluminators. In addition we also measured the emission spectra of our blue LED transilluminators with the filters in place.  We used the Ocean Optics USB2000 spectrophotometer for the measurements shown below.  Transmission spectra of the blue acrylic filter...

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