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Measuring emission spectra for the blue LED transilluminator

gel imaging transilluminator

Measuring emission spectra for the blue LED transilluminator

Our blue LED transilluminators use a 470 nm LED array in combination with blue and amber acrylic filters for viewing DNA bands in agarose gels stained with DNA dyes. Images of gels stained with Greenview, GelGreen and others can be seen in previous posts on our archived site here:  http://www.archive.iorodeo.com/blogs?tid=201 We recently used a Spectrophotometer to measure the transmission spectra of the blue and amber filters used in our transilluminators. In addition we also measured the emission spectra of our blue LED transilluminators with the filters in place.  We used the Ocean Optics USB2000 spectrophotometer for the measurements shown below.  Transmission spectra of the blue acrylic filter...

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Cyclic voltammetry testing with the new Potentiostat Shield

electrochemistry potentiostat

Cyclic voltammetry testing with the new Potentiostat Shield

In this blog post we provide data from our testing of the new Potentiostat Shield. We performed a side-by-side comparison between the Potentiostat Shield and the Cheapstat potentiostat using ascorbate cyclic voltammetry experiments. Ascorbate is a commonly used example of irreversible cyclic voltammetry (CV). This method can be used to determine the concentration of ascorbate in liquids such as orange juice as described in the originial Cheapstat paper by Rowe et al (2011).  Cyclic Voltammetry Methods We have previously posted a method for ascorbate cyclic voltammetry using the Cheapstat and screen printed carbon electrodes here. We repeated this test with a range of ascorbate concentrations (0.5 - 10 mM) with both the new Potentiostat Shield and the...

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IO Rodeo Open Source Potentiostat Shield

electrochemistry potentiostat

IO Rodeo Open Source Potentiostat Shield

We have recently been working on a new potentiostat shield. The potentiostat shield is an open source potentiostat for performing electrochemical measurements which is designed to work with the teensy 3.2 development board.  The potentiostat shield is easily programmed with the popular Arduino IDE (Teensyduino) which enables users to expand the design as it is easily hackable with popular, well-documented tools. Multiple voltage and current ranges making it a very flexible instrument.  Below is an image of a prototype (Teensy not shown). Hardware Some of the main features include: Designed as a shield for teensy 3.2 development board. 12-bit voltage output with four range...

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Potentiostat dummy cells

electrochemistry potentiostat

Potentiostat dummy cells

One of our most frequently asked questions on the Forums and via email is how to produce a calibration for the Cheapstat potentiostat. The Cheapstat potentiostat returns measured current values as raw unitless integers read from the ADC (analog to digital converter) at the output of the transimpedance amplifier (current to voltage converter). These integer values are proportional to the current flowing into the working electrode. To convert these unitless integer values to current measurements you need to use a calibration. This can be done using a dummy cell consisting of a single resistor of known value R.  We recently designed a potentiostat...

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Online Forum launched

Online Forum launched

We have launched a new online forum: http://forum.iorodeo.com.  Our aim in launching this Forum is that over time it will become a valuable resource for people interested in open source science equipment and DIYBio and grow to become a place to view frequently asked questions and discussions about our products. Hopefully it will also serve as a place to learn about us and what projects we are working on ! While we prefer and encourage general questions about our products on this public Forum, we do also still have a private Contact Form where you can get in touch with...

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